ziptip c4 (Millipore)
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ziptip c4
![( A ) NanoESI mass spectra of Aa RseP(WT) and Aa RseP(E18Q) obtained under “near-native” condition [200 mM ammonium acetate containing 0.032% (w/v) DDAO (pH 7.4)]. In Aa RseP(E18Q), peaks of # (11 + ~14+) and † (12 + ~15+) correspond to molecular masses of 52,303.1 ± 2.6 (+2965.4) and 57,625.3 ± 3.1 (+8287.6), respectively, indicating two different ligands were bound to the protein. ( B ) Upper panel shows the mass spectrum of isolated ligands from an acetone-precipitated Aa RseP(E18Q) sample using micro-reverse phase chromatography with <t>ZipTip</t> <t>C4.</t> It is likely that a slight amount of chemicals co-eluted with the ligand were observed at low m/z region. The bottom panel shows the MS/MS spectrum with the monoisotopic ligand mass [M+2H] 2+ at m/z 1483.71 indicated in green. The ligand was identified from the product ions as GFSIIAITALSVMMSVDFMVPDSHTLLA (M = 2965.50, monoisotopic), corresponding to Gly-266 through Ala-293 from CyoE, an endogenous E. coli enzyme. Observed b ions, y ions, and internal ions are indicated in red, blue and purple, respectively.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4173/pmc11864173/pmc11864173__sciadv.adu0925-f3.jpg)
Ziptip C4, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c4+ziptip/c4+ziptip/pmc11864173-365-15-17
Average 90 stars, based on 1 article reviews
Ziptip C4, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c4+ziptip/c4+ziptip/pmc11864173-365-15-17
Average 90 stars, based on 1 article reviews
ziptip c4 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state"
Article Title: Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state
Journal: Science Advances
doi: 10.1126/sciadv.adu0925
Figure Legend Snippet: ( A ) NanoESI mass spectra of Aa RseP(WT) and Aa RseP(E18Q) obtained under “near-native” condition [200 mM ammonium acetate containing 0.032% (w/v) DDAO (pH 7.4)]. In Aa RseP(E18Q), peaks of # (11 + ~14+) and † (12 + ~15+) correspond to molecular masses of 52,303.1 ± 2.6 (+2965.4) and 57,625.3 ± 3.1 (+8287.6), respectively, indicating two different ligands were bound to the protein. ( B ) Upper panel shows the mass spectrum of isolated ligands from an acetone-precipitated Aa RseP(E18Q) sample using micro-reverse phase chromatography with ZipTip C4. It is likely that a slight amount of chemicals co-eluted with the ligand were observed at low m/z region. The bottom panel shows the MS/MS spectrum with the monoisotopic ligand mass [M+2H] 2+ at m/z 1483.71 indicated in green. The ligand was identified from the product ions as GFSIIAITALSVMMSVDFMVPDSHTLLA (M = 2965.50, monoisotopic), corresponding to Gly-266 through Ala-293 from CyoE, an endogenous E. coli enzyme. Observed b ions, y ions, and internal ions are indicated in red, blue and purple, respectively.
Techniques Used: Isolation, Reversed-phase Chromatography, Tandem Mass Spectroscopy
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